Pattern formation mechanisms in motility mutants of Myxococcus xanthus

TL;DR

This study investigates how different motility mechanisms and reversal behaviors in Myxococcus xanthus lead to various spatial patterns, revealing that unidirectional motion causes large clusters and reversals produce mesh-like structures, driven by self-propulsion and steric interactions.

Contribution

It demonstrates that pattern formation in M. xanthus can arise from physical interactions alone, without biochemical signaling, and clarifies the roles of motility systems and reversal in spatial organization.

Findings

Unidirectional motility leads to large clusters and vortices.

Reversal behavior results in mesh-like structures.

Pattern formation is driven by self-propulsion and volume exclusion.

Abstract

Formation of spatial patterns of cells is a recurring theme in biology and often depends on regulated cell motility. Motility of M. xanthus depends on two motility machineries: the S-engine and A-engine. Moving M. xanthus cells can organize into spreading colonies or spore-filled fruiting bodies depending on their nutritional status. To understand these two pattern formation processes and the contributions by the two motility machineries, as well as cell reversal, we analyze spatial self-organization in 3 strains: i) a mutant that moves unidirectionally without reversing by the A-motility system only, ii) a unidirectional mutant that is also equipped with the S-motility system, and iii) the wild-type that, in addition to the two motility systems, reverses its direction of movement. The mutant moving by the A-engine illustrates that collective motion in the form of large moving clusters can arise in gliding bacteria due to steric interactions of the rod-shaped cells, without the need of invoking any biochemical signal regulation. The two-engine strain mutant reveals that the same phenomenon emerges when both motility systems are present, and as long as cells exhibit unidirectional motion only. From the study of these two strains, we conclude that unidirectional cell motion induces the formation of large moving clusters at low and intermediate densities, while it results into vortex formation at very high densities. These findings are consistent with what is known from self-propelled rods which strongly suggests that the combined effect of self-propulsion and volume exclusion interactions is the pattern formation mechanism leading to the observed phenomena. In addition, we learn that when cells reverse, as observed in the wild-type, cells form small but strongly elongated clusters and self-organize into a mesh-like structure at high enough densities.