Optical characterization of red blood cells from individuals with sickle cell trait and disease in Tanzania using quantitative phase imaging

TL;DR

This study uses quantitative phase imaging to analyze red blood cells from Tanzanian individuals with sickle cell trait and disease, enabling cost-effective, detailed cellular diagnostics in resource-limited settings.

Contribution

It introduces a cost-effective method using existing microscopes for detailed optical characterization of sickle cell affected cells in low-resource environments.

Findings

Successful optical measurements of RBCs from different health conditions

Transformation of standard microscopes into quantitative phase microscopes

Potential for improved diagnosis of sickle cell disease in low-resource settings

Abstract

Sickle cell disease (SCD) is common across Sub-Saharan Africa. However, the investigation of SCD in this area has been significantly limited mainly due to the lack of research facilities and skilled personnel. Here, we present optical measurements of individual red blood cells (RBCs) from healthy individuals and individuals with SCD and sickle cell trait in Tanzania using the quantitative phase imaging technique. By employing a quantitative phase imaging unit (QPIU), an existing microscope in a clinic is transformed into a powerful quantitative phase microscope providing measurements on the morphological, biochemical, and biomechanical properties of individual cells. The present approach will open up new opportunities for cost-effective investigation and diagnosis of several diseases in low resource environments.