Natural site-directed mutagenesis might exist in eukaryotic cells

TL;DR

This paper proposes that natural site-directed mutagenesis may occur in eukaryotic cells, driven by harmful agents and specific molecular mechanisms, challenging the view that it is solely a man-made technique.

Contribution

It introduces the novel hypothesis that natural site-directed mutagenesis exists in eukaryotic cells, based on experimental evidence and proposed molecular mechanisms.

Findings

Evidence suggests natural mutagenesis occurs in eukaryotic cells.

Mutagenesis is triggered by harmful agents.

Special transcription hotspots and intranuclear primers are involved.

Abstract

Site-directed mutagenesis refers to a man-made molecular biology method that is used to make genetic alterations in the DNA sequence of a gene of interest. But based on our recently published experimental findings, we propose that natural site-directed mutagenesis might exist in the eukaryotic cells, which is triggered by harmful agents and co-directed by special transcription hotspots and mutation-contained intranuclear primers.