Analytic and Numerical Models of Oxygen and Nutrient Diffusion, Metabolism Dynamics, and Architecture Optimization in Three-Dimensional Tissue Constructs with Applications and Insights in Cerebral Organoids

TL;DR

This paper develops analytic models for oxygen and nutrient diffusion in 3D tissue constructs, aiding design and understanding of tissue viability, especially in cerebral organoids, by overcoming limitations of numerical methods.

Contribution

It provides novel analytic solutions for steady-state and non-steady-state diffusion and metabolism in 3D tissues, applicable to various shapes and specifically applied to cerebral organoids.

Findings

Analytic solutions enable better prediction of cell viability.

Diffusion limitations can be mitigated by regional cell localization.

Models assist in designing tissue constructs with improved diffusion properties.

Abstract

Diffusion models are important in tissue engineering as they enable an understanding of molecular delivery to cells in tissue constructs. As three-dimensional (3D) tissue constructs become larger, more intricate, and more clinically applicable, it will be essential to understand internal dynamics and signaling molecule concentrations throughout the tissue. Diffusion characteristics present a significant limitation in many engineered tissues, particularly for avascular tissues and for cells whose viability, differentiation, or function are affected by concentrations of oxygen and nutrients. This paper seeks to provide novel analytic solutions for certain cases of steady-state and non-steady-state diffusion and metabolism in 3D construct designs (planar, cylindrical, and spherical forms), solutions that otherwise require mathematical approximations achieved through numerical methods. This model is applied to cerebral organoids, where it is shown that limitations in diffusion and organoid size can be partially overcome by localizing metabolically-active cells to an outer layer in a sphere, a regionalization process that is known to occur through neuroglial precursor migration both in organoids and in early brain development. The given prototypical solutions include a review of metabolic information for many cell types and can be broadly applied to many forms of tissue constructs. This work enables researchers to model oxygen and nutrient delivery to cells, predict cell viability, design constructs with improved diffusion capabilities, and accurately control molecular concentrations in tissue constructs that may be used in studying models of development and disease or for conditioning cells to enhance survival after insults like ischemia or implantation into the body, thereby providing a framework for better understanding and exploring the characteristics of engineered tissue constructs.