Living Wires - Effects of Size and Coating of Gold Nanoparticles in Altering the Electrical Properties of Physarum polycephalum and Lettuce Seedlings

TL;DR

This study investigates how gold nanoparticles affect the electrical properties of Physarum polycephalum and lettuce seedlings, demonstrating their potential for creating improved biological wires in biohybrid circuits.

Contribution

It provides new insights into nanoparticle uptake, toxicity, and electrical property modifications in P. polycephalum and lettuce, advancing biohybrid wiring technology.

Findings

Gold nanoparticles are internalized and assembled in vivo.

Electrical resistance decreases significantly in both organisms.

Lettuce seedlings show the greatest resistance reduction from 3MΩ to 0.5MΩ.

Abstract

The manipulation of biological substrates is becoming more popular route towards generating novel computing devices. Physarum polycephalum is used as a model organism in biocomputing because it can create `wires' for use in hybrid circuits; programmable growth by manipulation through external stimuli and the ability withstanding a current and its tolerance to hybridisation with a variety of nano/microparticles. Lettuce seedlings have also had previous interest invested in them for generating plant wires, although currently there is little information as to their suitability for such applications. In this study both P. polycephalum and Lettuce seedlings were hybridised with gold nanoparticles - functionalised and unfunctionalised - to explore their uptake, toxicological effects and, crucially, any alterations in electrical properties they bestow upon the organisms. Using various microscopy techniques it was shown that P. polycephalum and lettuce seedlings are able to internalize nanoparticles and assemble them in vivo, however some toxicological effects were observed. The electrical resistance of both lettuce seedlings and P. polycephalum was found to decrease, the most significant reduction being with lettuce seedlings whose resistance reduced from 3MOhms to 0.5MOhms. We conclude that gold is a suitable nanomaterial for biohybridisation specifically in creating conductive pathways for more efficient biological wires in self-growing hybrid circuitry.