A versatile method to generate multiple types of micropatterns

TL;DR

This paper introduces a flexible micropatterning method using plasma treatment, protein coating, and PLL-PEG backfill, capable of creating complex, multi-component, and gradient patterns on various substrates for cell research.

Contribution

The authors develop a versatile, simple protocol that is compatible with multiple materials and patterning strategies, enabling more elaborate cell microenvironments.

Findings

Produces homogeneous patterns on diverse substrates

Allows control of protein density and gradients

Enables creation of multi-component patterns in one step

Abstract

Micropatterning techniques have become an important tool for the study of cell behavior in controlled microenvironments. As a consequence, several approaches for the creation of micropatterns have been developed in recent years. However, the diversity of substrates, coatings and complex patterns used in cell science is so great that no single existing technique is capable of fabricating designs suitable for all experimental conditions. Hence, there is a need for patterning protocols that are flexible with regard to the materials used and compatible with different patterning strategies to create more elaborate setups. In this work, we present a versatile approach to micropatterning. The protocol is based on plasma treatment, protein coating, and a PLL-PEG backfill step, and produces homogeneous patterns on a variety of substrates. Protein density within the patterns can be controlled, and density gradients of surface-bound protein can be formed. Moreover, by combining the method with microcontact printing, it is possible to generate patterns composed of three different components within one iteration of the protocol. The technique is simple to implement and should enable cell science labs to create a broad range of complex and highly specialized microenvironments.