A one-piece 3D printed flexure translation stage for open-source microscopy

TL;DR

This paper presents a novel 3D printed monolithic flexure translation stage for microscopy, achieving sub-micron precision with minimal post-processing, high stability, and suitability for open-source, disposable, and parallel experiments.

Contribution

It introduces a fully 3D printed, monolithic flexure stage with sub-micron accuracy, low drift, and easy automation, advancing open-source hardware for scientific instruments.

Findings

Achieves less than 20 μm drift over a week without temperature stabilization.

Supports sub-micron-scale motion over an 8x8x4 mm range.

Can be automated with standard stepper motors.

Abstract

Open source hardware has the potential to revolutionise the way we build scientific instruments; with the advent of readily-available 3D printers, mechanical designs can now be shared, improved and replicated faster and more easily than ever before. However, printed parts are typically plastic and often perform poorly compared to traditionally machined mechanisms. We have overcome many of the limitations of 3D printed mechanisms by exploiting the compliance of the plastic to produce a monolithic 3D printed flexure translation stage, capable of sub-micron-scale motion over a range of $8\times8\times4\,$mm. This requires minimal post-print clean-up, and can be automated with readily-available stepper motors. The resulting plastic composite structure is very stiff and exhibits remarkably low drift, moving less than $20\,\mu$m over the course of a week, without temperature stabilisation. This enables us to construct a miniature microscope with excellent mechanical stability, perfect for timelapse measurements in situ in an incubator or fume hood. The ease of manufacture lends itself to use in containment facilities where disposability is advantageous, and to experiments requiring many microscopes in parallel. High performance mechanisms based on printed flexures need not be limited to microscopy, and we anticipate their use in other devices both within the laboratory and beyond.