Detection of basepair mismatches in DNA using graphene based nanopore device

TL;DR

This paper introduces a graphene nanopore device that detects DNA basepair mismatches by measuring electronic transport changes during DNA translocation, enabling accurate identification of both normal and mismatched pairs.

Contribution

The study presents a novel graphene-based nanopore method using Greens function approach for detecting DNA mismatches with high reliability, including non-standard base pairs.

Findings

Successfully detects AT and GC base pairs.

Identifies various mismatched base pairs.

Potential for sequencing other biomolecules.

Abstract

We present an unique way to detect basepair mismatches in DNA leading to different epigenetic disorder by the method of nanopore sequencing. Based on a tight-binding formulation of graphene nanopore based device, using Greens function approach we measure the changes in the electronic transport properties of the device as we translocate a double-stranded DNA through the nanopore embeded in a zigzag graphene nanoribbon. In the present work we not only successfully detect the usual AT and GC pairs, but also a set of possible mismatches in the complementary base-pairing without any ambiguity. Our investigation shows that this device can also be used for reliable sequential detection of other biomolecules.