Coherent motion of monolayer sheets under confinement and its pathological implications

TL;DR

This study models how epithelial cell sheets exhibit coherent rotation under confinement, revealing how physical parameters and perturbations like cell division influence tissue motion and potential pathological states.

Contribution

It introduces a cell-center based model demonstrating the emergence of coherent tissue rotation and explores how physical and biological factors affect this motion.

Findings

Coherent rotation arises from simple polarization-velocity alignment rules.

Physical parameters like cell density and stiffness dictate motion patterns.

Cell division can reverse the direction of tissue rotation.

Abstract

Coherent angular rotation of epithelial cells is thought to contribute to many vital physiological processes including tissue morphogenesis and glandular formation. However, factors regulating this motion, and the implications of this motion if perturbed remain incompletely understood. In the current study, we address these questions using a cell-center based model in which cells are polarized, motile, and interact with the neighboring cells via harmonic forces. We demonstrate that, a simple evolution rule in which the polarization of any cell tends to orient with its velocity vector can induce coherent motion in geometrically confined environments. In addition to recapitulating coherent rotational motion observed in experiments, our results also show the presence of radial movements and tissue behavior that can vary between solid-like and fluid-like. We show that the pattern of coherent motion is dictated by the combination of different physical parameters including number density, cell motility, system size, bulk cell stiffness and stiffness of cell-cell adhesions. We further observe that, perturbations in the form of cell division can induce a reversal in the direction of motion when cell division occurs synchronously. Moreover, when the confinement is removed, we see that the existing coherent motion leads to cell scattering, with bulk cell stiffness and stiffness of cell-cell contacts dictating the invasion pattern. In summary, our study provides an in-depth understanding of the origin of coherent rotation in confined tissues, and extracts useful insights into the influence of various physical parameters on the pattern of such movements