Recombinant transfer in the basic genome of E. coli

TL;DR

This study computationally analyzed the shared core genome of 32 E. coli strains, revealing pervasive homologous recombination, especially among closely related strains, driven likely by phage-mediated transduction, contributing to genetic diversity.

Contribution

It provides a detailed computational analysis of recombinant transfer patterns in the core genome of E. coli, highlighting the role of phages in genetic exchange.

Findings

Recombinant transfer is widespread across the E. coli core genome.

Recombinant segments often contain gene clusters with adaptive advantages.

Phage-mediated transduction likely facilitates genome-wide variability distribution.

Abstract

An approximation to the ~4 Mbp basic genome shared by 32 strains of E. coli representing six evolutionary groups has been derived and analyzed computationally. A multiple-alignment of the 32 complete genome sequences was filtered to remove mobile elements and identify the most reliable ~90% of the aligned length of each of the resulting 496 basic-genome pairs. Patterns of single-bp mutations (SNPs) in aligned pairs distinguish clonally inherited regions from regions where either genome has acquired DNA fragments from diverged genomes by homologous recombination since their last common ancestor. Such recombinant transfer is pervasive across the basic genome, mostly between genomes in the same evolutionary group, and generates many unique mosaic patterns. The six least-diverged genome-pairs have one or two recombinant transfers of length ~40-115 kbp (and few if any other transfers), each containing one or more gene clusters known to confer strong selective advantage in some environments. Moderately diverged genome-pairs (0.4-1% SNPs ) show mosaic patterns of interspersed clonal and recombinant regions of varying lengths throughout the basic genome, whereas more highly diverged pairs within an evolutionary group or pairs between evolutionary groups having >1.3% SNPs have few clonal matches longer than a few kbp. Many recombinant transfers appear to incorporate fragments of the entering DNA produced by restriction systems of the recipient cell. A simple computational model can closely fit the data. Most recombinant transfers seem likely to be due to generalized transduction by co-evolving populations of phages, which could efficiently distribute variability throughout bacterial genomes.