Holographic microscopy reconstruction in both object and image half spaces with undistorted 3D grid

TL;DR

This paper introduces a holographic microscopy reconstruction method that produces undistorted 3D grids in both object and image spaces, validated with high-resolution imaging of a USAF target.

Contribution

The proposed method enables undistorted 3D reconstruction in both object and image half spaces with a calibrated, orthogonal grid, improving accuracy in holographic microscopy.

Findings

Reconstruction yields an orthogonal, constant pixel pitch grid.

Method validated with USAF target imaging at various depths.

Reconstruction behaves as if in either object or image space.

Abstract

We propose an holographic microscopy reconstruction method, which propagates the hologram, in the object half space, in the vicinity of the object. The calibration yields reconstructions with an undistorted reconstruction grid i.e. with orthogonal $x$, $y$ and $z$ axis and constant pixels pitch. The method is validated with an USAF target imaged by a $\times$60 microscope objective, whose holograms are recorded and reconstructed for different USAF locations along the longitudinal axis: -75 to +75 $\mu$m. Since the reconstruction numerical phase mask, the reference phase curvature and MO form an afocal device, the reconstruction can be interpreted as occurring equivalently in the object or in image half space.