3-D refractive index tomograms and deformability of individual human red blood cells from cord blood of newborn infants and maternal blood

TL;DR

This study uses optical holographic micro-tomography to compare the 3-D refractive index, morphology, and deformability of red blood cells from newborns and adults, revealing significant biophysical differences and similarities.

Contribution

It provides a detailed, cell-level comparison of cord blood RBCs with adult RBCs using advanced 3-D imaging and dynamic measurements, highlighting unique properties of neonatal cells.

Findings

Cord RBCs are larger and more flattened than adult RBCs.

Cord RBCs have higher hemoglobin content and concentration.

Deformability of cord RBCs is similar to adult RBCs.

Abstract

Red blood cells (RBCs) from the cord blood of newborn infants have distinctive functions for fetal and infant development. To systematically investigate the biophysical characteristics of individual cord RBCs in newborn infants, a comparative study was performed of RBCs from cord blood of newborn infants, and of adult RBCs from mothers or non-pregnant women, employing optical holographic micro-tomography. Optical measurements of 3-D refractive index distributions, and of dynamic membrane fluctuations of individual RBCs, enabled retrieval of the morphological, biochemical, and mechanical properties of cord, maternal, and adult RBCs at the individual cell level. The volume and surface area of the cord RBCs were significant larger than those of RBCs from non-pregnant women, and cord RBCs have more flattened shapes than RBCs in adults. In addition, the Hb content in the cord RBCs of newborns was significantly greater. The Hb concentration in cord RBCs was higher than for non-pregnant women or maternal RBCs, but they were within the physiological range of adults. Interestingly, the amplitude of dynamic membrane fluctuations in cord RBCs were comparable to those in non-pregnant women and maternal RBCs, suggesting that the deformability of cord RBCs is similar to that of healthy RBCs in adults.