Enhancement of second-order nonlinear-optical signals by optical stimulation

TL;DR

This paper demonstrates a method to dramatically enhance second-order nonlinear optical signals using optical stimulation, significantly improving signal quality for bioimaging and interfacial studies.

Contribution

The authors introduce a stimulated enhancement technique for second-order nonlinear signals, providing a quantitative framework and experimental validation with SHG from collagen fibrils.

Findings

SHG signals enhanced >10^4 times with low fluence stimulation

Enhancement is most effective when spontaneous signals are weak

Technique improves signal-to-noise ratio in biological imaging

Abstract

Second-order nonlinear optical interactions such as sum- and difference-frequency generation are widely used for bioimaging and as selective probes of interfacial environments. However, inefficient nonlinear optical conversion often leads to poor signal-to-noise ratio and long signal acquisition times. Here, we demonstrate the dramatic enhancement of weak second-order nonlinear optical signals via stimulated sum- and difference-frequency generation. We present a conceptual framework to quantitatively describe the interaction and show that the process is highly sensitive to the relative optical phase of the stimulating field. To emphasize the utility of the technique, we demonstrate stimulated enhancement of second harmonic generation (SHG) from bovine collagen-I fibrils. Using a stimulating pulse fluence of only 3 nJ/cm2, we obtain an SHG enhancement >10^4 relative to the spontaneous signal. The stimulation enhancement is greatest in situations where spontaneous signals are the weakest - such as low laser power, small sample volume, and weak nonlinear susceptibility - emphasizing the potential for this technique to improve signal-to-noise ratios in biological imaging and interfacial spectroscopy.