Performance Analysis of Quantitative Phase Retrieval Method in Zernike Phase Contrast X-ray Microscopy

TL;DR

This paper evaluates a recently proposed quantitative phase retrieval method in Zernike phase contrast X-ray microscopy, analyzing its performance at different photon energies to optimize image contrast and accuracy for various sample types.

Contribution

The study provides a performance analysis of a new phase retrieval method across different photon energies, offering practical guidelines for its application in X-ray microscopy.

Findings

Optimal photon energy for weakly absorbing features is 2.5 keV.

Higher photon energies are better for strongly absorbing objects.

The method is effective over a wide energy range.

Abstract

Since the invention of Zernike phase contrast method in 1930, it has been widely used in optical microscopy and more recently in X-ray microscopy. Considering the image contrast is a mixture of absorption and phase information, we recently have proposed and demonstrated a method for quantitative phase retrieval in Zernike phase contrast X-ray microscopy. In this contribution, we analyzed the performance of this method at different photon energies. Intensity images of PMMA samples are simulated at 2.5 keV and 6.2 keV, respectively, and phase retrieval is performed using the proposed method. The results demonstrated that the proposed phase retrieval method is applicable over a wide energy range. For weakly absorbing features, the optimal photon energy is 2.5 keV, from the point of view of image contrast and accuracy of phase retrieval. On the other hand, in the case of strong absorption objects, a higher photon energy is preferred to reduce the error of phase retrieval. Those results can be used as guidelines to perform quantitative phase retrieval in Zernike phase contrast X-ray microscopy with the proposed method.