An in-vivo study of electrical charge distribution on the bacterial cell wall by Atomic Force Microscopy in vibrating force mode

TL;DR

This study introduces a novel in-vivo AFM method to map electrical charge distribution on bacterial cell walls with nanometer resolution, enhancing understanding of bacterial surface properties in physiological conditions.

Contribution

The paper presents an innovative AFM approach that measures surface stress variations to map electrical surface charge of bacteria in situ, improving resolution and applicability over existing electrostatic techniques.

Findings

Achieved spatial resolution better than few tens of nanometers.

Demonstrated feasibility of electrical surface charge mapping in physiological conditions.

Provided insights into bacterial adhesion and biofilm formation processes.

Abstract

We report an in-vivo electromechanical Atomic Force Microscopy (AFM) study of charge distribution on the cell wall of Gram plus Rhodococcus wratislaviensis bacteria, naturally adherent to a glass substrate, in physiological conditions. The method presented in this paper relies on a detailed study of AFM approach-retract curves giving the variation of the interaction force versus distance between tip and sample. In addition to classical height and mechanical (as stiffness) data, mapping of local electrical properties, as bacterial surface charge, was proved to be feasible at a spatial resolution better than few tens of nanometers. This innovative method relies on the measurement of the cantilever's surface stress through its deflection far from (higher than 10nm) the repulsive contact zone. The variations of surface stress come from modification of electrical surface charge of the cantilever (as in classical electrocapillary measurements) likely stemming from its charging during contact of both tip and sample electrical double layers. This method offers an important improvement in local electrical and electrochemical measurements at the solid-liquid interface particularly in high-molarity electrolytes when compared to technics focused on the direct use of electrostatic force. It thus opens a new way to directly investigate in-situ biological electrical surface processes involved in numerous practical and fundamental problems as bacterial adhesion, biofilm formation, microbial fuel cell, etc.