Reorganization of functionally connected brain subnetworks in high-functioning autism

TL;DR

This study investigates functional brain subnetworks in high-functioning autism during movie viewing, revealing specific reorganizations in the default-mode and VTL subnetworks that relate to symptom severity, highlighting the role of ventro-temporal and limbic systems.

Contribution

It identifies specific reorganization patterns in brain subnetworks in ASD and links them to behavioral symptom severity, using a novel combination of movie-based fMRI and graph theory analysis.

Findings

Differences in default-mode and VTL subnetworks between ASD and controls.

VTL subnetwork similarity correlates with autism severity scores.

Results are reproducible in an independent dataset.

Abstract

Background: Previous functional connectivity studies have found both hypo- and hyper-connectivity in brains of individuals having autism spectrum disorder (ASD). Here we studied abnormalities in functional brain subnetworks in high-functioning individuals with ASD during free viewing of a movie containing social cues and interactions. Methods: Thirteen subjects with ASD and 13 matched-pair controls watched a 68 minutes movie during functional magnetic resonance imaging. For each subject, we computed Pearson`s correlation between haemodynamic time-courses of each pair of 6-mm isotropic voxels. From the whole-brain functional networks, we derived individual and group-level subnetworks using graph theory. Scaled inclusivity was then calculated between all subject pairs to estimate intersubject similarity of connectivity structure of each subnetwork. Additional 27 individuals with ASD from the ABIDE resting-state database were included to test the reproducibility of the results. Results: Between-group differences were observed in the composition of default-mode and a ventro-temporal-limbic (VTL) subnetwork. The VTL subnetwork included amygdala, striatum, thalamus, parahippocampal, fusiform, and inferior temporal gyri. Further, VTL subnetwork similarity between subject pairs correlated significantly with similarity of symptom gravity measured with autism quotient. This correlation was observed also within the controls, and in the reproducibility dataset with ADI-R and ADOS scores. Conclusions: Reorganization of functional subnetworks in individuals with ASD clarifies the mixture of hypo- and hyper-connectivity findings. Importantly, only the functional organization of the VTL subnetwork emerges as a marker of inter-individual similarities that co-vary with behavioral measures across all participants. These findings suggest a pivotal role of ventro-temporal and limbic systems in autism.