Motor-free actin bundle contractility driven by molecular crowding

TL;DR

This study demonstrates that molecular crowding can induce significant contractile forces in actin bundles, reaching up to 3pN, which may contribute to biological force generation independently of chemical energy input.

Contribution

It extends the understanding of depletion forces from single filament pairs to multi-filament actin bundles, revealing higher forces and different dynamics.

Findings

Forces up to 3pN in actin bundles due to crowding

Exponential decay of forces with a 3.4s mean time

Crowding-induced forces may aid biological force generation

Abstract

Modeling approaches of suspended, rod-like particles and recent experimental data have shown that depletion forces display different signatures depending on the orientation of these particles. It has been shown that axial attraction of two rods yields contractile forces of 0.1pN that are independent of the relative axial shift of the two rods. Here, we measured depletion-caused interactions of actin bundles extending the phase space of single pairs of rods to a multi-particle system. In contrast to a filament pair, we found forces up to 3pN . Upon bundle relaxation forces decayed exponentially with a mean decay time of 3.4s . These different dynamics are explained within the frame of a mathematical model by taking pairwise interactions to a multi-filament scale. The macromolecular content employed for our experiments is well below the crowding of cells. Thus, we propose that arising forces can contribute to biological force generation without the need to convert chemical energy into mechanical work.