A Brief Comparison Between Available Bio-printing Methods

TL;DR

This paper reviews and compares laser-assisted and laser-free 3D bioprinting methods, focusing on resolution, speed, cell density, and viability to evaluate their suitability for tissue engineering and medical applications.

Contribution

It provides a comprehensive comparison of current bioprinting techniques, highlighting their advantages and limitations in resolution and speed.

Findings

Laser-assisted methods have higher resolution but slower speed.

Laser-free methods are faster with lower resolution.

Trade-offs exist between resolution, speed, and cell viability.

Abstract

The scarcity of organs for transplant has led to large waiting lists of very sick patients. In drug development, the time required for human trials greatly increases the time to market. Drug companies are searching for alternative environments where the in-vivo conditions can be closely replicated. Both these problems could be addressed by manufacturing artificial human tissue. Recently, researchers in tissue engineering have developed tissue generation methods based on 3-D printing to fabricate artificial human tissue. Broadly, these methods could be classified as laser-assisted and laser free. The former have very fine spatial resolutions (10s of $\mu$m) but suffer from slow speed ( $< 10^2$ drops per second). The later have lower spatial resolutions (100s of $\mu$ m) but are very fast (up to $5\times 10^3$ drops per second). In this paper we review state-of-the-art methods in each of these classes and provide a comparison based on reported resolution, printing speed, cell density and cell viability.