Patterned and Functionalized Nanofiber Scaffolds in Three-Dimensional Hydrogel Constructs Enhance Neurite Outgrowth and Directional Control

TL;DR

This study demonstrates that patterned, functionalized nanofiber scaffolds within 3D hydrogels can effectively direct and enhance neurite outgrowth, advancing neural tissue engineering and regenerative strategies.

Contribution

It introduces a novel combination of aligned, biofunctionalized nanofibers and hydrogels to control neurite growth in 3D neural tissue constructs.

Findings

Nanofiber alignment controls neurite direction in 2D and 3D cultures.

Laminin-functionalized nanofibers significantly increase neurite length.

The system enables creation of complex 3D neural tissue models.

Abstract

Neural tissue engineering holds incredible potential to restore functional capabilities to damaged neural tissue. It was hypothesized that patterned and functionalized nanofiber scaffolds could control neurite direction and enhance neurite outgrowth. Aligned nanofibers were created according to a mathematical model and were shown to enable significant control over the direction of neurite outgrowth in both two-dimensional (2D) and three-dimensional (3D) neuronal cultures. Laminin-functionalized nanofibers in 3D hyaluronic acid (HA) hydrogels enabled significant alignment of neurites with nanofibers, enabled significant neurite tracking of nanofibers, and significantly increased the distance over which neurites could extend. This work demonstrates the ability to create unique 3D neural tissue constructs using a combined system of hydrogel and nanofiber scaffolding. Importantly, patterned and biofunctionalized nanofiber scaffolds that can control direction and increase length of neurite outgrowth in three-dimensions hold much potential for neural tissue engineering. This approach offers advancements in the development of implantable neural tissue constructs that enable control of neural development and reproduction of neuroanatomical pathways, with the ultimate goal being the achievement of functional neural regeneration.