Salinity tolerance in plants: attempts to manipulate ion transport

TL;DR

This review explores ion transport mechanisms in plants, emphasizing how manipulating ion channels and transporters can enhance salinity tolerance, with insights from both natural plant differences and synthetic biology approaches.

Contribution

It provides a comprehensive analysis of ion transport principles, compares salt-tolerant and sensitive plants, and discusses novel genetic and synthetic biology strategies for improving salinity tolerance.

Findings

Ion transport is crucial for plant salinity tolerance.

Manipulating ion channels and transporters offers potential for crop improvement.

Synthetic biology approaches open new avenues for enhancing plant stress resilience.

Abstract

Ion transport is the major determining factor of salinity tolerance in plants. A simple scheme of a plant cell with ion fluxes provides basic understanding of ion transport and the corresponding changes of ion concentrations under salinity. The review describes in detail basic principles of ion transport for a plant cell, introduces set of transporters essential for sodium and potassium uptake and efflux, analyses driving forces of ion transport and compares ion fluxes measured by several techniques. Study of differences in ion transport between salt tolerant halophytes and salt-sensitive plants with an emphasis on transport of potassium and sodium via plasma membranes offers knowledge for increasing salinity tolerance. Effects of salt stress on ion transport properties of membranes show huge opportunities for manipulating ion transport. Several attempts to overexpress or knockout ion transporters for changing salinity tolerance are described. Future perspectives are questioned with more attention given to potential candidate ion channels and transporters for altered expression. The potential direction of increasing salinity tolerance by modifying ion channels and transporters is discussed and questioned. An alternative approach from synthetic biology is to modify the existing membrane transport proteins or create new ones with desired properties for transforming agricultural crops. The approach had not been widely used earlier and leads also to theoretical and pure scientific aspects of protein chemistry, structure-function relations of membrane proteins, systems biology and physiology of stress and ion homeostasis.