Simultaneous Multi-Harmonic Imaging of Nanoparticles in Tissues for Increased Selectivity

TL;DR

This paper presents a novel multi-harmonic imaging method using Bismuth Ferrite nanoparticles for enhanced tumor tissue detection and mapping in biological samples.

Contribution

It introduces a new imaging protocol based on simultaneous second and third harmonic generation for improved selectivity in tissue imaging.

Findings

Efficient generation of second and third harmonic signals by BFO nanoparticles.

Enhanced tissue imaging selectivity through co-localization of harmonic signals.

Potential application of BFO nanoparticles as reference markers in microscopy.

Abstract

We investigate the use of Bismuth Ferrite (BFO) nanoparticles for tumor tissue labelling in combination with infrared multi-photon excitation at 1250 nm. We report the efficient and simultaneous generation of second and third harmonic by the nanoparticles. On this basis, we set up a novel imaging protocol based on the co-localization of the two harmonic signals and demonstrate its benefits in terms of increased selectivity against endogenous background sources in tissue samples. Finally, we discuss the use of BFO nanoparticles as mapping reference structures for correlative light-electron microscopy.