Adsorption at Liquid Interfaces Induces Amyloid Fibril Bending and Ring Formation

TL;DR

This study reveals that protein fibrils at liquid interfaces develop spontaneous curvature leading to ring formation, influenced by fibril thickness and molecular structure, with implications for biological processes and material design.

Contribution

It demonstrates that amyloid fibrils at interfaces exhibit spontaneous curvature and ring formation, a novel insight into fibril behavior influenced by molecular and environmental factors.

Findings

Fibrils show non-Gaussian curvature distribution at interfaces.

Spontaneous curvature can lead to stable ring structures.

Fibril thickness affects ring formation propensity.

Abstract

Protein fibril accumulation at interfaces is an important step in many physiological processes and neurodegenerative diseases as well as in designing materials. Here we show, using $\beta$-lactoglobulin fibrils as a model, that semiflexible fibrils exposed to a surface do not possess the Gaussian distribution of curvatures characteristic for wormlike chains, but instead exhibit a spontaneous curvature, which can even lead to ring-like conformations. The long-lived presence of such rings is confirmed by atomic force microscopy, cryogenic scanning electron microscopy and passive probe particle tracking at air- and oil-water interfaces. We reason that this spontaneous curvature is governed by structural characteristics on the molecular level and is to be expected when a chiral and polar fibril is placed in an inhomogeneous environment such as an interface. By testing $\beta$-lactoglobulin fibrils with varying average thicknesses, we conclude that fibril thickness plays a determining role in the propensity to form rings.