DNA hairpins primarily promote duplex melting rather than inhibiting hybridization

TL;DR

This study uses a coarse-grained DNA model to show that hairpins mainly promote duplex melting by stabilizing partially melted states, significantly accelerating dissociation, which informs DNA device design.

Contribution

It reveals that DNA hairpins primarily facilitate melting rather than inhibiting hybridization, providing quantitative insights into their effects on DNA duplex dynamics.

Findings

Hairpins reduce hybridization rates by factors of 2 and 10.

Hairpins accelerate melting rates by factors of ~100 and ~2000.

Hairpins stabilize partially melted states, aiding dissociation.

Abstract

The effect of secondary structure on DNA duplex formation is poorly understood. We use a coarse-grained model of DNA to show that specific 3- and 4-base pair hairpins reduce hybridization rates by factors of 2 and 10 respectively, in good agreement with experiment. By contrast, melting rates are accelerated by factors of ~100 and ~2000. This surprisingly large speed-up occurs because hairpins form during the melting process, stabilizing partially melted states, and facilitating dissociation. These results may help guide the design of DNA devices that use hairpins to modulate hybridization and dissociation pathways and rates.