Effects of low-level deuterium enrichment on bacterial growth

TL;DR

This study precisely measures how minimal deuterium enrichment affects E. coli growth, revealing a threshold at 0.5% D and suggesting ultralow enrichment may promote bacterial adaptation, possibly due to isotopic resonance.

Contribution

It identifies the specific deuterium concentration threshold affecting bacterial growth and supports the hypothesis of isotopic resonance influencing microbial adaptation.

Findings

Adverse effects appear above 0.5% D enrichment.

Ultralow enrichment (up to 0.25% D) may enhance bacterial growth.

Bacterial adaptation favors ultralow deuterium levels.

Abstract

Using very precise (up to 0.05%) measurements of the growth parameters for bacteria E. coli grown on minimal media, we aimed to determine the lowest deuterium concentration at which the adverse effects that are prominent at higher enrichments start to become noticeable. Such a threshold was found at 0.5% D, a surprisingly high value, while the ultralow deuterium concentrations (up to 0.25% D) showed signs of the opposite trend. Bacterial adaptation for 400 generations in isotopically different environment confirmed preference for ultralow (up to 0.25% D) enrichment. This effect appears to be similar to those described in sporadic but multiple earlier reports. Possible explanations include hormesis and isotopic resonance phenomena, with the latter explanation being favored.