Rheotaxis facilitates upstream navigation of mammalian sperm cells

TL;DR

This study demonstrates that mammalian sperm cells use rheotaxis, a fluid flow response, to navigate upstream, with experimental and theoretical evidence showing how shear flow influences their swimming behavior.

Contribution

First quantitative experimental analysis of mammalian sperm rheotaxis, revealing how shear flow and flagellar dynamics enable upstream navigation.

Findings

Sperm exhibit stable upstream spiraling motion due to rheotaxis

Fluid shear and flagellar chirality are key to sperm navigation

A minimal model accurately describes sperm rheotactic behavior

Abstract

A major puzzle in biology is how mammalian sperm determine and maintain the correct swimming direction during the various phases of the sexual reproduction process. Whilst chemotaxis is assumed to dominate in the immediate vicinity of the ovum, it is unclear which biochemical or physical cues guide spermatozoa on their long journey towards the egg cell. Currently debated mechanisms range from peristaltic pumping to temperature sensing (thermotaxis) and direct response to fluid flow variations (rheotaxis), but little is known quantitatively about their relative importance. Here, we report the first quantitative experimental study of mammalian sperm rheotaxis. Using microfluidic devices, we investigate systematically the swimming behavior of human and bull sperm over the whole range of physiologically relevant shear rates and viscosities. Our measurements show that the interplay of fluid shear, steric surface-interactions and chirality of the flagellar beat leads to a stable upstream spiraling motion of sperm cells, thus providing a generic and robust rectification mechanism to support mammalian fertilisation. To rationalise these findings, we identify a minimal mathematical model that is capable of describing quantitatively the experimental observations. The combined experimental and theoretical evidence supports the hypothesis that the shape and beat patterns of mammalian sperm cells have evolved to optimally exploit rheotaxis for long-distance navigation.