A Smartphone Controlled Handheld Microfluidic Liquid Handling System

TL;DR

This paper introduces a compact, smartphone-controlled handheld microfluidic system capable of automating complex liquid handling tasks for biochemical assays, enabling portable and integrated lab-on-a-chip applications.

Contribution

The development of a lightweight, battery-powered handheld microfluidic platform that automates liquid handling without external infrastructure, integrating elastomeric valves and a pneumatic system.

Findings

Successfully automated immunoassay steps on a portable device

Device weighs 829g and operates for over 8 hours on a single charge

Potential applications include various biochemical and diagnostic assays

Abstract

Microfluidics and lab-on-a-chip technologies have made it possible to manipulate small volume liquids with unprecedented resolution, automation and integration. However, most current microfluidic systems still rely on bulky off-chip infrastructures such as compressed pressure sources, syringe pumps and computers to achieve complex liquid manipulation functions. Here, we present a handheld automated microfluidic liquid handling system controlled by a smartphone, which is enabled by combining elastomeric on-chip valves and a compact pneumatic system. As a demonstration, we show that the system can automatically perform all the liquid handling steps of a bead-based sandwich immunoassay on a multi-layer PDMS chip without any human intervention. The footprint of the system is 6 by 10.5 by 16.5cm, and the total weight is 829g including battery. Powered by a 12.8V 1500mAh Li battery, the system consumed 2.2W on average during the immunoassay and lasted for 8.7 hrs. This handheld microfluidic liquid handling platform is generally applicable to many biochemical and cell-based assays requiring complex liquid manipulation and sample preparation steps such as FISH, PCR, flow cytometry and nucleic acid sequencing. In particular, the integration of this technology with read-out biosensors may help enable the realization of the long-sought Tricorder-like handheld in-vitro diagnostic (IVD) systems.