Improved 3D Superresolution Localization Microscopy Using Adaptive Optics
Nicolas Piro, Thomas Pengo, Nicolas Olivier, Suliana Manley
TL;DR
This paper introduces a novel 3D super-resolution microscopy technique that employs adaptive optics with a deformable mirror to precisely shape the point spread function, enhancing imaging of microtubules.
Contribution
The work presents a new adaptive optics method for 3D super-resolution microscopy, enabling continuous and controllable PSF shaping for improved imaging.
Findings
Successful 3D STORM imaging of microtubules.
Enhanced control over the point spread function.
Demonstrated versatility of the deformable mirror approach.
Abstract
We demonstrate a new versatile method for 3D super-resolution microscopy by using a deformable mirror to shape the point spread function of our microscope in a continuous and controllable way. We apply this for 3D STORM imaging of microtubules.
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Taxonomy
TopicsAdvanced Fluorescence Microscopy Techniques · Digital Holography and Microscopy · Near-Field Optical Microscopy