Selection and orientation of different particles in single particle imaging

TL;DR

This paper demonstrates that the correlation maximization method can effectively select identical particles and determine their orientations in single particle imaging experiments using X-ray free electron lasers.

Contribution

It introduces the application of the correlation maximization method for selecting identical particles and determining their orientations from mixed samples in single particle imaging.

Findings

The method successfully identifies identical particles in mixed samples.

It accurately determines particle orientations.

The approach improves data quality in single particle imaging experiments.

Abstract

The short pulses of X-ray free electron lasers can produce diffraction patterns with structural information before radiation damage destroys the particle. The particles are injected into the beam in random orientations and they should be identical. However, in real experimental conditions it is not always possible to have identical particles. In this paper we show that the correlation maximization method, developed earlier, is able to select identical particles from a mixture and find their orientations simultaneously.