Quantitative analysis of intra-Golgi transport reveals inter-cisternal exchange for all cargo

TL;DR

This study uses a quantitative framework to analyze intra-Golgi transport, providing evidence for inter-cisternal exchange in transporting various cargo, while acknowledging the potential role of cisternal progression.

Contribution

The paper introduces a comprehensive quantitative model that supports inter-cisternal exchange in Golgi transport for all cargo types, challenging previous models.

Findings

Inter-cisternal exchange occurs for small and large cargo.

Transport involves membrane structures larger than vesicles.

Current data cannot exclude cisternal progression as a contributing mechanism.

Abstract

The mechanisms controlling the transport of proteins across the Golgi stack of mammalian and plant cells is the subject of intense debate, with two models, cisternal progression and inter-cisternal exchange, emerging as major contenders. A variety of transport experiments have claimed support for each of these models. We reevaluate these experiments using a single quantitative coarse-grained framework of intra-Golgi transport that accounts for both transport models and their many variants. Our analysis makes a definitive case for the existence of inter-cisternal exchange both for small membrane proteins (VSVG) and large protein complexes (procollagen) -- this implies that membrane structures larger than the typical protein-coated vesicles must be involved in transport. Notwithstanding, we find that current observations on protein transport cannot rule out cisternal progression as contributing significantly to the transport process. To discriminate between the different models of intra-Golgi transport, we suggest experiments and an analysis based on our extended theoretical framework that compare the dynamics of transiting and resident proteins.