Locomotion of microspheres for super-resolution imaging

TL;DR

This paper introduces a simple method to control the position and movement of microspheres using a glass micropipette, enabling super-resolution imaging at arbitrary 3D points and tracking moving objects, with broad applications in microscopy.

Contribution

It presents a novel, straightforward technique for positioning and moving microspheres, enhancing super-resolution imaging capabilities in practical microscopy applications.

Findings

Enables sub-diffraction imaging at arbitrary 3D points

Allows tracking of moving objects with microspheres

Provides a practical method for microsphere control

Abstract

Super-resolution imaging using sub-diffraction field localization by micron sized transparent beads (microspheres) was recently demonstrated [1]. Practical applications in microscopy require control over the positioning of the microspheres. We present a simple method of positioning and controllable movement of a microsphere by using a glass micropipette. This allows sub-diffraction imaging at arbitrary points in three dimensions, as well as the ability to track moving objects. The results are relevant to a broad scope of applications, including sample inspection, and bio-imaging.