Extended Linear Response for Bioanalytical Applications Using Multiple Enzymes

TL;DR

This paper presents a new framework for extending the linear response range of biosensors by using two enzymes with different kinetics, demonstrated with lysine detection, and provides optimization methods for future bioanalytical systems.

Contribution

It introduces a novel approach combining two enzymes to enhance linear response range and offers optimization techniques for bioanalytical applications.

Findings

Extended linear response range achieved for lysine detection.

Analyzed enzyme kinetics to elucidate response extension mechanism.

Developed optimization methods for enzyme-based biosensors.

Abstract

We develop a framework for optimizing a novel approach to extending the linear range of bioanalytical systems and biosensors by utilizing two enzymes with different kinetic responses to the input chemical as their substrate. Data for the flow-injection amperometric system devised for detection of lysine based on the function of L-Lysine-alpha-Oxidase and Lysine-2-monooxygenase are analyzed. Lysine is a homotropic substrate for the latter enzyme. We elucidate the mechanism for extending the linear response range and develop optimization techniques for future applications of such systems.