Evolution of intratumoral phenotypic heterogeneity: the role of trait inheritance

TL;DR

This paper models tumor heterogeneity by simulating how phenotypic traits like proliferation and migration evolve over time through inheritance and environmental interactions, revealing complex dynamics in tumor growth.

Contribution

It introduces an off-lattice agent-based model focusing on trait inheritance and environmental effects to study tumor heterogeneity evolution.

Findings

Trait inheritance influences heterogeneity dynamics

Multiple phenotypic combinations can coexist in tumors

Environmental constraints shape trait distribution over time

Abstract

A tumor can be thought of as an ecosystem, which critically means that we cannot just consider it as a collection of mutated cells but more as a complex system of many interacting cellular and microenvironmental elements. At its simplest, a growing tumor with increased proliferation capacity must compete for space as a limited resource. Hypercellularity leads to a contact-inhibited core with a competitive proliferating rim. Evolution and selection occurs, and an individual cell's capacity to survive and propagate is determined by its combination of traits and interaction with the environment. With heterogeneity in phenotypes, the clone that will dominate is not always obvious as there are both local interactions and global pressures. Several combinations of phenotypes can coexist, changing the fitness of the whole. To understand some aspects of heterogeneity in a growing tumor we build an off-lattice agent based model consisting of individual cells with assigned trait values for proliferation and migration rates. We represent heterogeneity in these traits with frequency distributions and combinations of traits with density maps. How the distributions change over time is dependent on how traits are passed on to progeny cells, which is our main inquiry. We bypass the translation of genetics to behavior by focussing on the functional end result of inheritance of the phenotype combined with the environmental influence of limited space.