Synthetic Biology in Leishmaniasis: Design,simulation and validation of constructed Genetic circuit

TL;DR

This paper presents the design, simulation, and validation of a synthetic genetic circuit targeting a specific enzyme in Leishmania, demonstrating its dynamic behaviors and robustness, which is novel for protozoan parasites.

Contribution

It introduces the first synthetic genetic circuit for a protozoan parasite, with detailed simulation and validation showing its functional properties.

Findings

Circuit exhibits responsiveness, oscillatory, and bistable behaviors.

Circuit demonstrates intrinsic robustness.

Validation confirms effective design and function.

Abstract

Building circuits and studying their behavior in cells is a major goal of systems and synthetic biology. Synthetic biology enables the precise control of cellular states for systems studies, the discovery of novel parts, control strategies, and interactions for the design of robust synthetic systems. To the best of our knowledge,there are no literature reports for the synthetic circuit construction for protozoan parasites. This paper describes the construction of genetic circuit for the targeted enzyme inositol phosphorylceramide synthase belonging to the protozoan parasite Leishmania. To explore the dynamic nature of the circuit designed, simulation was done followed by circuit validation by qualitative and quantitative approaches. The genetic circuit designed for inositol phosphorylceramide synthase shows responsiveness, oscillatory and bistable behavior, together with intrinsic robustness.