Gold particle quantification in immuno-gold labelled sections for transmission electron microscopy

TL;DR

This paper presents an algorithm for accurately quantifying gold particles in immuno-TEM images, improving the analysis of specific binding in biological tissue samples.

Contribution

It introduces a novel algorithm that enhances the quantification accuracy of gold particles in immuno-TEM, based on existing protocols.

Findings

Improved accuracy in gold particle quantification.

Algorithm aligns with existing immuno-labeling protocols.

Facilitates better analysis of biological samples.

Abstract

We briefly outline an algorithm for accurate quantification of specific binding of gold particles to fixed biological tissue samples prepared for immuno-transmission electron microscopy (TEM). The algorithm is based on existing protocols for rational accounting of colloidal gold particles used in secondary antibodies for immuno-gold labeling.