Actomyosin contractility rotates the cell nucleus

TL;DR

This study demonstrates that actomyosin contractility induces persistent rotational motion of the cell nucleus, influenced by cell shape and actin dynamics, with implications for nuclear homeostasis.

Contribution

It provides a hydrodynamic model linking actomyosin contractility to nuclear rotation, supported by experimental validation in fibroblast cells.

Findings

Nuclei rotate persistently on circular and triangular patterns.

Nuclear rotation speed varies with cell shape and actin contractility.

Actin flow profiles match hydrodynamic model predictions.

Abstract

The nucleus of the eukaryotic cell functions amidst active cytoskeletal filaments, but its response to the stresses carried by these filaments is largely unexplored. We report here the results of studies of the translational and rotational dynamics of the nuclei of single fibroblast cells, with the effects of cell migration suppressed by plating onto fibronectin-coated micro-fabricated patterns. Patterns of the same area but different shapes and/or aspect ratio were used to study the effect of cell geometry on the dynamics. On circles, squares and equilateral triangles, the nucleus undergoes persistent rotational motion, while on high-aspect-ratio rectangles of the same area it moves only back and forth. The circle and the triangle showed respectively the largest and the smallest angular speed. We show that our observations can be understood through a hydrodynamic approach in which the nucleus is treated as a highly viscous inclusion residing in a less viscous fluid of orientable filaments endowed with active stresses. Lowering actin contractility selectively by introducing blebbistatin at low concentrations drastically reduced the speed and persistence time of the angular motion of the nucleus. Time-lapse imaging of actin revealed a correlated hydrodynamic flow around the nucleus, with profile and magnitude consistent with the results of our theoretical approach. Coherent intracellular flows and consequent nuclear rotation thus appear to be a generic property that cells must balance by specific mechanisms in order to maintain nuclear homeostasis.