Functionalized AFM probes for force spectroscopy: eigenmodes shape and stiffness calibration through thermal noise measurements

TL;DR

This study investigates how attaching a colloidal bead to an AFM cantilever affects its eigenmodes and stiffness calibration, using thermal noise measurements and an analytical model for improved quantitative force spectroscopy.

Contribution

It provides a detailed analysis of eigenmode shape changes due to bead loading and introduces a model for accurate stiffness calibration of functionalized AFM probes.

Findings

First eigenmode shape remains nearly unchanged by mass loading.

Higher mode nodes shift towards the free end with a bead attached.

Analytical model accurately predicts thermal noise amplitudes and enables precise stiffness calibration.

Abstract

The functionalization of an Atomic Force Microscope (AFM) cantilever with a colloidal bead is a widely used technique when the geometry between the probe and the sample must be controlled, particularly in force spectroscopy. But some questions remain: how does a bead glued at the end of a cantilever influence its mechanical response ? And more important for quantitative measurements, can we still determine the stiffness of the AFM probe with traditional techniques? In this article, the influence of a colloidal mass loading on the eigenmodes shape and resonant frequency is investigated by measuring the thermal noise on rectangular AFM microcantilevers with and without a bead attached at their extremities. The experiments are performed with a home-made ultra-sensitive AFM, based on differential interferometry. The focused beam from the interferometer probes the cantilever at different positions and the spatial shapes of the modes are determined up to the fifth resonance, without external excitation. The results clearly demonstrate that the first eigenmode almost doesn't change by mass loading. However the oscillation behavior of higher resonances present a marked difference: with a particle glued at its extremity, the nodes of the mode are displaced towards the free end of the cantilever. These results are compared to an analytical model taking into account the mass and the inertial moment of the load in an Euler-Bernoulli framework, where the normalization of the eigenmodes is explicitly worked out in order to allow a quantitative prediction of the thermal noise amplitude of each mode. A good agreement between the experimental results and the analytical model is demonstrated, allowing a clean calibration of the probe stiffness.