OpenSPIM - an open access platform for light sheet microscopy
Peter Pitrone, Johannes Schindelin, Luke Stuyvenberg, Stephan, Preibisch, Michael Weber, Kevin W. Eliceiri, Jan Huisken, Pavel Tomancak
TL;DR
OpenSPIM provides detailed instructions and an open platform for building and sharing light sheet microscopy systems, making advanced imaging technology accessible and customizable for developmental biology research.
Contribution
It introduces an open hardware and software platform for SPIM, enabling widespread access and customization of light sheet microscopy.
Findings
OpenSPIM is freely accessible online.
It enables live imaging of entire embryos.
The platform is customizable for various applications.
Abstract
Light sheet microscopy promises to revolutionize developmental biology by enabling live in toto imaging of entire embryos with minimal phototoxicity. We present detailed instructions for building a compact and customizable Selective Plane Illumination Microscopy (SPIM) system. The integrated OpenSPIM hardware and software platform is shared with the scientific community through a public website, thereby making light sheet microscopy accessible for widespread use and optimization to various applications.
Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.