Ciliary contact interactions dominate surface scattering of swimming eukaryotes

TL;DR

This study reveals that direct ciliary contact interactions, rather than hydrodynamic forces, primarily govern how swimming eukaryotes scatter from surfaces, enabling the design of microfluidic devices for controlling microorganism movement.

Contribution

It provides the first direct experimental evidence that ciliary contact dominates surface scattering in eukaryotic microorganisms, informing microfluidic control strategies.

Findings

Ciliary contact interactions are the main factor in surface scattering.

Optimal microfluidic ratchets can be designed for microorganism rectification.

Results are applicable across diverse eukaryotic species.

Abstract

Interactions between swimming cells and surfaces are essential to many microbiological processes, from bacterial biofilm formation to human fertilization. However, in spite of their fundamental importance, relatively little is known about the physical mechanisms that govern the scattering of flagellated or ciliated cells from solid surfaces. A more detailed understanding of these interactions promises not only new biological insights into structure and dynamics of flagella and cilia, but may also lead to new microfluidic techniques for controlling cell motility and microbial locomotion, with potential applications ranging from diagnostic tools to therapeutic protein synthesis and photosynthetic biofuel production. Due to fundamental differences in physiology and swimming strategies, it is an open question whether microfluidic transport and rectification schemes that have recently been demonstrated for pusher-type microswimmers such as bacteria and sperm cells, can be transferred to puller-type algae and other motile eukaryotes, as it is not known whether long-range hydrodynamic or short-range mechanical forces dominate the surface interactions of these microorganisms. Here, using high-speed microscopic imaging, we present direct experimental evidence that the surface scattering of both mammalian sperm cells and unicellular green algae is primarily governed by direct ciliary contact interactions. Building on this insight, we predict and verify experimentally the existence of optimal microfluidic ratchets that maximize rectification of initially uniform Chlamydomonas reinhardtii suspensions. Since mechano-elastic properties of cilia are conserved across eukaryotic species, we expect that our results apply to a wide range of swimming microorganisms.