Sea Spider development: How the encysting Anoplodactylus eroticus matures from a buoyant nymph to a grounded adult

TL;DR

This study provides a detailed description of the development of the sea spider Anoplodactylus eroticus from hatchling to adult, revealing key morphological changes and addressing evolutionary questions about arthropod body plan origins.

Contribution

It offers the first comprehensive documentation of post-embryonic development in A. eroticus using multiple microscopy techniques, challenging previous assumptions about pycnogonid development and arthropod evolution.

Findings

Larvae burrow in hydroids and undergo morphogenesis

Eight developmental stages before maturity

Chelifores are present and mobile throughout development

Abstract

In order to understand how animals evolved over time, biologists must learn how their body parts form during their development. The following is a detailed description of how one species of sea spider transforms from a hatchling to an 8-legged adult. Pycnogonids, or sea spiders, comprise a primitive lineage of arthropods. As such, they hold potential to reveal insights into arthropod evolution. Recent phylogenetic analyses have supported their position as either basal chelicerates or as a separate, fifth major lineage of extant arthropods. Disagreements concerning pycnogonid relations to other arthropods is partly a product of too few primary observations of pycnogonid anatomy and development. This investigation of post-embryonic development of the pycnogonid Anoplodactylus eroticus employs multiple techniques of anatomical observation, including Nomarski optics, scanning electron microscopy and fluorescence microscopy, in order to thoroughly document the life cycle. After the second post-embryonic stage, larvae of A. eroticus burrow within a hydroid and undergo morphogenesis. Larvae emerge from the hydroid and simultaneously molt into the juvenile stage. Over the course of post-embryonic development there are eight stages preceding the mature adult. All structures, except for the anteriormost appendages, the chelifores, undergo some degree of transformation. Chelifores are present prior to hatching and remain mobile over the course of development. Some larger issues important to arthropod evolution are addressed, such as the equivalent of a germband and labrum in pycnogonids. Post-embryonic development of A. eroticus provides an example counteracting previous reports of anamorphic development and a four-segmented head in the pycnogonid ground pattern, findings that were extrapolated to fit the ground pattern of Arthropoda.