A Simple Laser-Based Device for Simultaneous Microbial Culture and Absorbance Measurement

TL;DR

This paper introduces a simple laser-based device capable of simultaneously measuring microbial growth and absorbance in up to 16 samples, facilitating real-time monitoring for microbiology research.

Contribution

The novel AMC-d device allows semi-continuous absorbance measurement during microbial cultivation, improving efficiency and data collection in microbiological studies.

Findings

Successfully measured growth curves of E. coli and Haloferax volcanii.

Demonstrated simultaneous measurement of up to 16 samples.

Enabled real-time monitoring of microbial growth.

Abstract

In this work we present a device specifically designed to study microbial growth with several applications related to environmental microbiology and other areas of research as astrobiology. The Automated Measuring and Cultivation device (AMC-d) enables semi-continuous absorbance measurements directly during cultivation. It can measure simultaneously up to 16 samples. Growth curves using low and fast growing microorganism were plotted, including: Escherichia coli, and Haloferax volcanii, an halophilic archaeon.