Protein Inference and Protein Quantification: Two Sides of the Same Coin

TL;DR

This paper explores whether protein inference can be improved by treating it as a special case of protein quantification, using simple quantification methods to achieve competitive results in mass spectrometry data analysis.

Contribution

The paper proposes viewing protein inference as a subset of protein quantification and demonstrates that simple quantification methods can effectively solve inference problems.

Findings

Quantification methods are competitive with existing inference algorithms.

Protein inference can be approached as a quantification problem.

Opened new avenues for developing inference algorithms from a quantification perspective.

Abstract

Motivation: In mass spectrometry-based shotgun proteomics, protein quantification and protein identification are two major computational problems. To quantify the protein abundance, a list of proteins must be firstly inferred from the sample. Then the relative or absolute protein abundance is estimated with quantification methods, such as spectral counting. Until now, researchers have been dealing with these two processes separately. In fact, they are two sides of same coin in the sense that truly present proteins are those proteins with non-zero abundances. Then, one interesting question is if we regard the protein inference problem as a special protein quantification problem, is it possible to achieve better protein inference performance? Contribution: In this paper, we investigate the feasibility of using protein quantification methods to solve the protein inference problem. Protein inference is to determine whether each candidate protein is present in the sample or not. Protein quantification is to calculate the abundance of each protein. Naturally, the absent proteins should have zero abundances. Thus, we argue that the protein inference problem can be viewed as a special case of protein quantification problem: present proteins are those proteins with non-zero abundances. Based on this idea, our paper tries to use three very simple protein quantification methods to solve the protein inference problem effectively. Results: The experimental results on six datasets show that these three methods are competitive with previous protein inference algorithms. This demonstrates that it is plausible to take the protein inference problem as a special case of protein quantification, which opens the door of devising more effective protein inference algorithms from a quantification perspective.