On the Way to More Convenient Description of Drug-Plasma Protein Binding

TL;DR

This paper derives a formula to calculate free drug concentration over time after a single application, based on plasma protein binding parameters, aiding in more convenient drug binding analysis.

Contribution

It introduces a new formula that estimates free drug concentration from total concentration and binding parameters, simplifying plasma protein binding analysis.

Findings

Derived a formula for free drug concentration over time

Allows calculation of free drug levels from total concentration and binding constants

Provides a theoretical basis for more convenient drug-protein binding assessment

Abstract

The theoretical case is considered where the total amount of plasma protein is conserved, but the drug is eliminated after its single application. After a single drug application at time t = 0, the total drug concentration is measured at times ti,...,tk and the total drug concentration at time ti is denoted by {\phi}i. Our discussion is limited to one protein binding site. The quantity of plasma protein ({\Lambda}), the association constant (Ka) and the total concentration of the drug {\phi}i at time ti are considered as independent variables. Free drug concentration, plasma protein bound concentration and free drug fraction are given as functions of these "new" variables. The aim of this communication is to derive the formula that allows to calculate the free drug concentration at any time after the drug application, based on 3 parameters: the association constant of the drug, the total plasma concentration of the drug and the concentration of the protein. If the plasma protein quantity ({\Lambda}) and the association constant (Ka) are known, then from the knowledge of the total drug concentration {\phi}i at time ti it is possible to determine the free drug concentration at time ti.