Immunomodulatory role of an Ayurvedic formulation on imbalanced immune-metabolics during inflammatory responses of obesity and pre-diabetic disease

TL;DR

This study investigates an Ayurvedic formulation, Kal-1, demonstrating its potential to modulate immune responses and improve metabolic parameters in diet-induced obese and pre-diabetic mice, suggesting therapeutic benefits for obesity-related inflammation.

Contribution

The paper provides novel evidence that Kal-1 can reduce obesity-related inflammation and metabolic disturbances in mice, highlighting its immunomodulatory effects in obesity and pre-diabetes.

Findings

Kal-1 reduced body weight and fat in obese mice.

Kal-1 improved blood glucose, insulin, triglycerides, and cholesterol levels.

Kal-1 modulated cytokine and adipokine levels, indicating immune regulation.

Abstract

Obesity and related type 2 diabetes are associated with a low level chronic inflammatory state with disease status increasing at epidemic proportions worldwide. It is now universally accepted that the underlying immune-inflammatory responses mediated within the adipose tissue in obesity are central to the development of disease. Once initiated, chronic inflammation associated with obesity leads to the modulation of immune cell function. In the present study we aimed to investigate the effect of an ayurvedic formulation (named as Kal-1, an abbreviation derived from the procuring source) on diet-induced obesity and type II diabetes using C57BL/6J mice. The study was planned into two groups using obese and pre-diabetic mouse model. The mice were fed on high-fat with increased diet and, different amounts (5, 20 and 75 {\mu}g) of Kal-1 were given with disease progression for 21 weeks in first group whereas mice were first put on the same diet for 21 weeks and then treated with same amounts of Kal-1. A significant reduction in body weight and fat pads, fasting blood glucose levels, insulin levels and biochemical parameters like triglycerides and cholesterol were observed in obese and diabetic mice+Kal-1 than control (lean) mice fed on low fat diet, though the optimum amounts of Kal-1 were 20 and 75 {\mu}g in first and second group respectively. A noteworthy immunological correction in important readouts viz. resistin, leptin, HMW adiponection and an array of pro- & anti-cytokines (IL-4, IL-10, IL-1{\alpha}, IL-1{\beta}, IL-4, IL-6, IL-10, TNF-{\alpha} and MCP-1) was also observed in both the groups with the mentioned amount of Kal-1. We conclude that Kal-1 has immunomodulatory potential for diet-induced obesity and associated metabolic disorders.