BioFET-SIM Web Interface: Implementation and Two Applications

TL;DR

The paper introduces a web interface for BioFET-SIM, enabling easier setup of simulations and demonstrating its use through two case studies involving peptide orientation and antibody-antigen interactions on nanowires.

Contribution

It provides a user-friendly web interface for BioFET-SIM and showcases its application in modeling nanowire biosensor interactions.

Findings

Orientation of peptides affects sensor sensitivity.

Antibody/antigen binding influences nanowire sensor response.

Web interface facilitates understanding of experimental data.

Abstract

We present a web interface for the BioFET-SIM program. The web interface allows to conveniently setup calculations based on the BioFET-SIM multiple charges model. As an illustration, two case studies are presented. In the first case, a generic peptide with opposite charges on both ends is inverted in orientation on a semiconducting nanowire surface leading to a corresponding change in sign of the computed sensitivity of the device. In the second case, the binding of an antibody/antigen complex on the nanowire surface is studied in terms of orientation and analyte/nanowire surface distance. We demonstrate how the BioFET-SIM web interface can aid in the understanding of experimental data and postulate alternative ways of antibody/antigen orientation on the nanowire surface.