Soluble oligomerization provides a beneficial fitness effect on destabilizing mutations

TL;DR

This study reveals that soluble oligomerization of destabilized DHFR proteins in E. coli at high temperature enhances fitness by counteracting destabilization and preventing aggregation, despite mutations.

Contribution

It introduces a new site-specific mutagenesis technique and demonstrates how soluble oligomerization can mitigate destabilizing mutations in vivo.

Findings

Mutations in DHFR do not significantly affect catalytic activity or fitness within limited ranges.

Protein stability correlates with intracellular abundance, influenced by homeostatic mechanisms.

At high temperature, oligomerization of destabilized proteins increases, improving cell fitness.

Abstract

Mutations create the genetic diversity on which selective pressures can act, yet also create structural instability in proteins. How, then, is it possible for organisms to ameliorate mutation-induced perturbations of protein stability while maintaining biological fitness and gaining a selective advantage? Here we used a new technique of site-specific chromosomal mutagenesis to introduce a selected set of mostly destabilizing mutations into folA - an essential chromosomal gene of E. coli encoding dihydrofolate reductase (DHFR) - to determine how changes in protein stability, activity and abundance affect fitness. In total, 27 E.coli strains carrying mutant DHFR were created. We found no significant correlation between protein stability and its catalytic activity nor between catalytic activity and fitness in a limited range of variation of catalytic activity observed in mutants. The stability of these mutants is strongly correlated with their intracellular abundance; suggesting that protein homeostatic machinery plays an active role in maintaining intracellular concentrations of proteins. Fitness also shows a significant correlation with intracellular abundance of soluble DHFR in cells growing at 30oC. At 42oC, on the other hand, the picture was mixed, yet remarkable: a few strains carrying mutant DHFR proteins aggregated rendering them nonviable, but, intriguingly, the majority exhibited fitness higher than wild type. We found that mutational destabilization of DHFR proteins in E. coli is counterbalanced at 42oC by their soluble oligomerization, thereby restoring structural stability and protecting against aggregation.