Multi-plectoneme phase of double-stranded DNA under torsion

TL;DR

This paper models DNA supercoiling using the worm-like chain model, revealing a multi-plectoneme phase that explains experimental data across various conditions and clarifies the torque behavior in plectonemic DNA.

Contribution

It introduces a comprehensive theoretical framework that captures the multi-plectoneme phase in DNA supercoiling, resolving previous model shortcomings without adjustable parameters.

Findings

The model reproduces experimental data over a broad range of conditions.

Identifies the multi-plectoneme phase as a key factor in DNA supercoiling behavior.

Shows non-constant torque in the plectonemic phase.

Abstract

We use the worm-like chain model to study supercoiling of DNA under tension and torque. The model reproduces experimental data for a much broader range of forces, salt concentrations and contour lengths than previous approaches. Our theory shows, for the first time, how the behavior of the system is controlled by a multi-plectoneme phase in a wide range of parameters. This phase does not only affect turn-extension curves but also leads to a non-constant torque in the plectonemic phase. Shortcomings from previous models and inconsistencies between experimental data are resolved in our theory without the need of adjustable parameters.