Extracellular electrical signals in a neuron-surface junction: model of heterogeneous membrane conductivity

TL;DR

This paper introduces a theoretical model of heterogeneous membrane conductivity to explain the diverse extracellular signals recorded from neurons, emphasizing the inhomogeneous distribution of potential and charge near ion channels.

Contribution

It proposes a novel mathematical model accounting for membrane heterogeneity and integrates it into an electrical circuit to analyze extracellular signal variations.

Findings

Heterogeneous membrane conductivity influences extracellular signal shapes.

Variations in junction parameters affect signal amplitude and waveform.

The model explains diverse extracellular recordings through membrane inhomogeneity.

Abstract

Signals recorded from neurons with extracellular planar sensors have a wide range of waveforms and amplitudes. This variety is a result of different physical conditions affecting the ion currents through a cellular membrane. The transmembrane currents are often considered by macroscopic membrane models as essentially a homogeneous process. However, this assumption is doubtful, since ions move through ion channels, which are scattered within the membrane. Accounting for this fact, the present work proposes a theoretical model of heterogeneous membrane conductivity. The model is based on the hypothesis that both potential and charge are distributed inhomogeneously on the membrane surface, concentrated near channel pores, as the direct consequence of the inhomogeneous transmembrane current. A system of continuity equations having non-stationary and quasi-stationary forms expresses this fact mathematically. The present work performs mathematical analysis of the proposed equations, following by the synthesis of the equivalent electric element of a heterogeneous membrane current. This element is further used to construct a model of the cell-surface electric junction in a form of the equivalent electrical circuit. After that a study of how the heterogeneous membrane conductivity affects parameters of the extracellular electrical signal is performed. As the result it was found that variation of the passive characteristics of the cell-surface junction, conductivity of the cleft and the cleft height, could lead to different shapes of the extracellular signals.