Effects of electropermeabilization and gene electrotransfert on the lateral mobility of a GPi anchored protein

TL;DR

This study investigates how electropermeabilization and gene electrotransfert influence the lateral mobility of a GPi anchored protein, Rae-1, revealing membrane disruptions and DNA aggregation effects.

Contribution

It demonstrates that electropermeabilization and gene electrotransfert significantly alter membrane protein mobility and cause DNA to form large aggregates, providing new insights into cellular membrane dynamics.

Findings

10-20% of membrane surface occupied by pores

Electropermeabilization reduces Rae-1 mobility

DNA insertion excludes Rae-1 from membrane areas

Abstract

Electropermeabilization is a method by which molecules can be introduced in living cells through the plasma membrane. Here, we show that electropermeabilization affects the lateral mobility of Rae-1, a GPi anchored protein. Our results shows that 10 to 20 % of the membrane surface is occupied by defaults or pores. Gene electrotransfert also affects the lateral mobility of Rae-1. Furthermore, we clearly show that once inserted into the plasma membrane, DNA totaly excludes Rae-1, indicating that DNA molecules are tightly packed together to forms huge aggregates.