Enol form of uracil in the GU "wobble" pair. NMR evidence
Denis A. Semenov, Vladimir A. Reznikov
TL;DR
This paper provides NMR evidence that uracil can adopt an enol form in GU wobble pairs, challenging traditional keto-based models and offering insights into RNA base pairing dynamics.
Contribution
It presents direct NMR evidence for the enol form of uracil in GU wobble pairs, a novel insight into RNA base pairing mechanisms.
Findings
Uracil can exist in enol form within GU pairs.
NMR spectroscopy can detect enolization in RNA bases.
GU wobble pairs can mimic GC pairing due to enolization.
Abstract
GU "wobble" pair may contain either the enol form of uracil and the keto form of guanine or the enol form of guanine and the keto form of uracil. A direct proof of uracil existence in enol form can be obtained by using NMR spectroscopy. In HNN-COSY experiments the GU pair imitates the GC pair due to the enolization of uracil.
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Taxonomy
TopicsRNA and protein synthesis mechanisms · DNA and Nucleic Acid Chemistry · Enzyme Structure and Function