Two Hands Are Better Than One (up to constant factors)
Sarah Cannon, Erik D. Demaine, Martin L. Demaine, Sarah Eisenstat,, Matthew J. Patitz, Robert Schweller, Scott M. Summers, Andrew Winslow

TL;DR
This paper compares seeded and seedless two-handed tile self-assembly models, demonstrating the greater power of the latter in simulating systems, constructing shapes, and the complexity of verification.
Contribution
It shows how to simulate seeded systems with two-handed systems, constructs shapes with separation in tile complexity, and proves verification is co-NP-complete in the two-handed model.
Findings
Two-handed model can simulate seeded systems with only constant factor increase.
Finite shapes exhibit a busy-beaver separation in tile complexity.
Verification of unique assembly is co-NP-complete in the two-handed model.
Abstract
We study the difference between the standard seeded model of tile self-assembly, and the "seedless" two-handed model of tile self-assembly. Most of our results suggest that the two-handed model is more powerful. In particular, we show how to simulate any seeded system with a two-handed system that is essentially just a constant factor larger. We exhibit finite shapes with a busy-beaver separation in the number of distinct tiles required by seeded versus two-handed, and exhibit an infinite shape that can be constructed two-handed but not seeded. Finally, we show that verifying whether a given system uniquely assembles a desired supertile is co-NP-complete in the two-handed model, while it was known to be polynomially solvable in the seeded model.
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